DOG RECOMBINANT 5-HT2A RECEPTOR
MULTISCREEN™ STABLE CELL LINES
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Full-length Dog HTR2A cDNA (GenBank Accession Number NM_001005869.1) with FLAG-tag sequence at the N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin
Background: 5-HT2A (5-hydroxytryptamine receptor 2A) is a receptor for serotonin. It is expressed throughout the central nervous system in the neocortex and olfactory tubercle. Additionally, it is expressed in platelets, fibroblasts and neurons of the peripheral nervous system. 5-HT2A receptor agonists may have important clinical value in the treatment of various disorders, such as depression, anxiety, bipolar disorder and schizophrenia. It is also a receptor for the human polyomavirus, JC virus.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Bonaventure et al. (2005) Molecular and pharmacological characterization of serotonin 5-HT2A and 5-HT2B receptor subtypes in dog. Eur J Pharmacol 513(3):181-92.
Meltzer and Li (2003) Serotonin receptors: their key role in drugs to treat schizophrenia. Prog Neuropsychopharmacol Biol Psychiatry 27:1159-1172.
Stam et al. (1992) Genomic organization, coding sequence and functional expression of human 5-HT2 and HT1A receptor genes. Eur J Pharmacol 227:158-162.