HUMAN RECOMBINANT 5-HT1B RECEPTOR
MULTISCREEN™ STABLE CELL LINES
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human HTR1B cDNA (GenBank accession number NM_000863.1) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin
Stability in progress
Background: The human serotonin receptor 5-HT1B is a G protein-coupled receptor. 5-HT1B receptors are present in many parts of the central nervous system, but most notably, can be found in the basal ganglia, striatum, and frontal cortex. 5-HT1B receptors inhibit the release of many neurotransmitters, such as serotonin, GABA, acetylcholine, and glutamate. 5-HT1B ligands may prove to be therapeutic in the treatment of various disorders such as depression, anxiety, and aggression.
Application: Functional assays
Figure 1. Dose-dependent inhibition of forskolin stimulated intracellular cAMP accumulation upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Hamblin et al. (1992) Molecular cloning and functional characterization of a human 5- HT1B serotonin receptor: a homologue of the rat 5-HT1B receptor with 5-HT1D-like pharmacological specificity. Biochem Biophys Res Commun, 184:752-759.
Hamon et al. (1990) The main features of central 5-HT1 receptors. Neuropsychopharmacology 3:349-360.
Ruf et al. (2009) The 5-HT(1B) receptor: a novel target for the pathophysiology of depression. Curr Drug Targets 10):1118-1138.