HUMAN RECOMBINANT 5-HT1A RECEPTOR
MULTISCREEN™ DIVISION ARRESTED CELL LINE
1 vial (4 x 106) frozen cells
Cell Banker 2 (Amsbio)
Expression vector containing full-length human HTR1A cDNA (GenBank accession number NM_000524.2) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS
Background: The human serotonin receptor 5-HT1A is a G protein-coupled receptor. 5-HT1A receptors can be found mainly in the cerebral cortex, hippocampus, septum, amygdala, and raphe nucelus, with lesser amounts in the basal ganglia and thalamus. 5-HT1A ligands may prove to be therapeutic in the treatment of various disorders such as depression, anxiety, and schizophrenia.
Application: Functional assays
Figure Legend. Dose-dependent inhibition of forskolin-stimulated intracellular cAMP accumulation upon treatment with ligand, measured with MULTISCREENTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01).
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