MULTISCREEN™ STABLE CELL LINES

HUMAN RECOMBINANT β1 ADRENERGIC RECEPTOR

Product Information

Catalog Number:
H1437

Lot Number:
H1437-090814

Quantity:
1 vial (2 x 106) frozen cells

Freeze Medium:
Sigma Freezing Medium (C-6164)

Host cell:
HEK293T

Transfection:
Expression vector containing full-length human ADRB1 cDNA (GenBank Accession Number AF169007.1) with FLAG tag sequence at N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM/F12, 10% FBS, 800 μg/mL G418

Stability:
Stable in culture for minimum of two months

Data Sheet

Background: Norepinephrine is implicated in a wide range of physiological processes through activation of nine different G-protein-coupled receptors (α1a, α1b, α1d, α2a, α2b, α2c, ß1, ß2, ß3). The human ß1-adrenergic receptor is a 477–amino acid protein found in various heart and brain tissues. ß1 has an important role in the contractile action of valves in cardiac and digestive systems.

Application: Functional assays

Figure 1. Dose-dependent stimulation of pERK level upon treatment with ligand, monitored with FlexStation (Cisbio 64AERPEG). Figure 2. Dose-dependent increase of intracellular cAMP level upon treatment with ligand, measured with cAMP HiRange kit (Cisbio 62AM6PEC). Figure 3. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor- expressing cells.

References:

Oostendorp et al. (2000) Contribution of beta-adrenoceptor subtypes to relaxtion of colon and oesophagus and pacemaker activity of ureter in wildtype and beta(3)-adrenoceptor knockout mice. Br J Pharmacol 130:747-758.

Sato et al. (1996) Molecular characterization of pharmacological properties of T-0509 for beta-adrenoceptors. Eur J Pharmacol 315:363-367.

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