MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT AT1 RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human AGTR1 cDNA (same as GenBank Accession Number NM_000685.4 except for one silent mutation) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 µg/mL puromycin
Stable in culture for minimum of two months
Background: AT1 (Angiotensin II, type 1) receptor is an angiotensin receptor with vasopressor effects and regulates aldosterone secretion. It is essential in controlling blood pressure and volume in the cardiovascular system. AT1 receptor antagonists are drugs useful in the treatment of hypertension, diabetic nephropathy and congestive heart failure. Blockade of AT1 receptors could also be a novel approach in the treatment of mood disorders and neurodegenerative diseases of the brain.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, monitored with FlexStation. Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Bergsma et al. (1992) Cloning and characterization of a human angiotensin II type 1 receptor. Biochem Biophys Res Commun 183:989-995.
Jagadeesh (1998) Angiotensin II receptors-antagonists, molecular biology, and signal transduction. Indian J Exp Biol 36:1171-1194.
Saavedra et al. (1998) Blockade of brain angiotensin II AT1 receptors ameliorates stress, anxiety, brain inflammation and ischemia: Therapeutic implications. Psychoneuroendocrinology 36:1-18.