MULTISCREENTM STABLE CELL LINE
RAT RECOMBINANT A1 RECEPTOR
Catalog Number: Cr1427
Lot Number: Cr1427-070121
Quantity: 1 vial (2 x 106) frozen cells
Freeze Medium: CellBanker2
Host cell: HEK293T
Transfection: Expression vector containing full-length rat ADORA1 cDNA (GenBank Accession Number NM_017155.3) with FLAG tag sequence at N-terminus
Recommended Storage: Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin
Stability: In progress
Background: Adenosine A1 receptor (ADORA1 or RDC7) is a G protein- coupled receptor for adenosine. RT-PCR detected A1 expression in the brain, colon, jejunum, ileum and kidney. A1 functions in sleep-wake regulation and the fertilization process.
Application: Functional assays
Figure 1. Dose-dependent inhibition of forskolin-stimulated intracellular cAMP accumulation upon treatment with ligand, measured with MultiScreenTM TR- FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti- FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Ethier and Madison (2006) Adenosine A1 receptors mediate mobilization of calcium in human bronchial smooth muscle cells. Am J Respir Cell Mol Biol 35:496-502.