MULTISCREEN™ STABLE CELL LINES
MOUSE RECOMBINANT EBI2 RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length mouse EBI2 cDNA (GenBank Accession Number NM_183031.2) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM-F12, 10% FBS, 10 μg/mL puromycin
Background: Epstein-Barr virus-induced gene 2 (EBI2, also known as GPR183) is a GPCR required for humoral immune responses and polymorphisms have been associated with inflammatory autoimmune diseases. It is expressed in B-lymphocyte cell lines as well as lymphoid tissues but not T-lymphocyte cell lines or peripheral blood T-lymphocytes. 7α,25-dihydroxycholesterol (7α,25-OHC) and other oxysterols act as chemoattractants for immune cells expressing EBI2 by directing cell migration. Mice deficient in cholesterol 25- hydroxylase (CH25H, required for generation of 7α,25-OHC) fail to generate EBI2 biological activity in vivo and shows that the EBI2-oxysterol signaling pathway plays an important role in the adaptive immune response.
Application: Functional assays
Figure 1. Dose-dependent cAMP accumulation upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Hannedouche et al. (2011) Oxysterols direct immune cell migration via EBI2. Nature 475:524-527
Birkenbach et al. (1993) Epstein-Barr virus-induced genes: first lymphocyte-specific G Protein-coupled peptide receptors. J Virol. 67(4):2209-2220.