MULTISCREEN™ STABLE CELL LINES
DOG RECOMBINANT GNRH RECEPTOR
Catalog Number: Cd1283
Lot Number: Cd1283-030121
Quantity: 1 vial (2 x 106) frozen cells
Freeze Medium: Cellbanker 2
Host cell: HEK293T
Transfection: Expression vector containing full-length dog GNRHR cDNA (GenBank Accession Number NM_001003121.1.) with FLAG tag sequence at N-terminus
Recommended Storage: Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin
Stability: In progress
Background: Gonadotropin-releasing hormone receptor (GnRHR) is a receptor for gonadotropin-releasing hormone (GnRH), also known as luteinizing hormone- releasing hormone (LHRH) and prolactin release-inhibiting factor (PIF). GnRHR is expressed on the surface of pituitary gonadotrope cells as well as lymphocytes, breast, ovary, and prostate. Gametes and preimplantation embryos also express GnRH and GnRHR. Binding of GnRH to GnRHR causes the release of follicle stimulating hormone (FSH) and luteinizing hormone (LH) from gonadotropic cells of the anterior pituitary. Mutations in GnRHR may cause hypogonadotropic hypogonadism and may be associated with anosmia.
Application: Functional assays
Figure 1. Dose-dependent calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Receptor Expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Adelman et al. Two mammalian genes transcribed from opposite strands of the same DNA locus. Science 235:1514-1517, 1987.
Adelman et al. Isolation of the gene and hypothalamic cDNA for the common precursor of gonadotropin-releasing hormone and prolactin release-inhibiting factor in human and rat. Proc Nat Acad Sci 83:179-183, 1986.