MULTISCREEN™ STABLE CELL LINES

DOG RECOMBINANT B2 RECEPTOR

Product Information

Catalog Number:
Cd1199

Lot Number:
Cd1199-031516

Quantity:
1 vial (2 x 106) frozen cells

Freeze Medium:
Sigma Freezing Medium (C-6164)

Host cell:
HEK293T

Transfection:
Expression vector containing full-length dog B2 cDNA (GenBank Accession Number NM_001003095.1) with FLAG tag sequence at N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin

Stability:
In progress

Data Sheet

Background:

Bradykinin receptor B2 is a constitutively expressed G protein-coupled receptor. In canines, recent studies revealed that the canine heart can be preconditioned by an abdominal surgical incision to mediate a protective effect through the activation of the B2 receptor. In the presence of the B2 antagonist HOE140, the protective effect was not observed. Thus, B2 antagonists may be a promising new class of compounds to treat ischemic heart disease or for clinical use after the onset of cerebral ischemia.

Application: Functional assays

Figure 1. Dose-dependent calcium flux upon treatment with ligand, monitored with FLIPR. Figure 2. Dose-dependent increase of intracellular cAMP level upon treatment with ligand. Figure 3. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.

References:

 

Gross, G. J., Baker, J. E., Moore, J., Falck, J. R., & Nithipatikom, K. (2011). Abdominal Surgical Incision Induces Remote Preconditioning of Trauma (RPCT) via Activation of Bradykinin Receptors (BK2R) and the Cytochrome P450 Epoxygenase Pathway in Canine Hearts. Cardiovascular Drugs and Therapy, 25(6), 517–522.

Heitsch (2003) The therapeutic potential of bradykinin B2 receptor agonists in the treatment of cardiovascular disease. Expert Opin Investig Drugs 12:759-770.

Jones, W. K., Fan, G.-C., Liao, S., Zhang, J.-M., Wang, Y., Weintraub, N. L., … Ren, X. (2009). Peripheral Nociception Associated With Surgical Incision Elicits Remote Nonischemic Cardioprotection Via Neurogenic Activation of Protein Kinase C Signaling. Circulation, 120(11 Suppl), S1–S9.

FOR RESEARCH USE ONLY.
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