MULTISCREEN™ STABLE CELL LINES

GUINEA PIG RECOMBINANT B2 RECEPTOR

Product Information

Catalog Number:
Cc1199

Lot Number:
Cc1199-031516

Quantity:
1 vial (2 x 106) frozen cells

Freeze Medium:
Sigma Freezing Medium (C-6164)

Host cell:
HEK293T

Transfection:
Expression vector containing full-length guinea pig B2 cDNA (GenBank Accession Number XM_013144370) with FLAG tag sequence at N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin

Stability:
Stability in progress

Data Sheet

Background: Bradykinin receptor B2 is a constitutively expressed G protein-coupled receptor. In guinea pigs, the B2 receptor is expressed in the small intestine on a majority of the ganglion cells in the submucosal and myenteric plexuses. The activation of the B2 receptor by the ligand bradykinin stimulates neurogenic chloride secretion in the guinea pig ileum through COX increasing PGE2 production. Recent studies on pregnant guinea pigs, revealed the importance B2 plays in regulating blood pressure and trophoblast invasion during placental development. These results suggest that B2 may be a promising target for treating abnormal placentation and related disorders.

Application: Functional assays

Figure 1. Dose-dependent calcium flux upon treatment with ligand, monitored with FLIPR. Figure 2. Dose-dependent increase of intracellular cAMP level upon treatment with ligand. Figure 3. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.

References:

Kawaguchi, A., Sato, M., Kimura, M., Yamazaki, T., Yamamoto, H., Tazaki, M., … Shibukawa, Y. (2015). Functional expression of bradykinin B1 and B2receptors in neonatal rat trigeminal ganglion neurons. Frontiers in Cellular Neuroscience, 9, 229. http://doi.org/10.3389/fncel.2015.00229

Qu, M.-H., Ji, W.-S., Zhao, T.-K., Fang, C.-Y., Mao, S.-M., & Gao, Z.-Q. (2016). Neurophysiological mechanisms of bradykinin-evoked mucosal chloride secretion in guinea pig small intestine. World Journal of Gastrointestinal Pathophysiology, 7(1), 150–159.

Valdés, G., Schneider, D., Corthorn, J., Ortíz, R., Acuña, S., & Padilla, O. (2014). Administration of angiotensin II and a bradykinin B2 receptor blocker in midpregnancy impairs gestational outcome in guinea pigs. Reproductive Biology and Endocrinology : RB&E, 12, 49. http://doi.org/10.1186/1477-7827-12-49

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