MULTISCREEN™ β-ARRESTIN2 STABLE CELL LINE
HUMAN RECOMBINANT β2 ADRENERGIC RECEPTOR
Catalog Number: CA1438BA2
Lot Number: CA1438BA2-090221
Quantity: 1 vial (2 x 106) frozen cells
Freeze Medium: CellBanker 2 (Amsbio 11891)
Host cell: HEK293T β-Arrestin2
Transfection: Expression vector containing full-length human ADRB2 cDNA (GenBank Accession Number NM_000024) with FLAG tag sequence at N-terminus and and ARRB2 cDNA (GenBank Accession Number NM_004313.3)
Recommended Storage: Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 µg/mL puromycin, 50 µg/mL hygromycin
Stability: In progress
Background: Norepinephrine is implicated in a wide range of physiological processes through activation of nine different G-protein-coupled receptors (α1a, α1b, α1d, α2a, α2b, α2c, ß1, ß2, ß3). The human ß2-adrenergic receptor was the first 7- transmembrane receptor for a hormone or neurotransmitter to have its crystal structure solved. It has been suggested that the β2-adrenoceptor may form homodimers as well as oligomers with other receptors. The β2-adrenoceptor mediates the actions of catecholamines in multiple tissues. They are responsible for relaxation of vascular, uterine, and airway smooth muscle, and are involved in metabolic and endocrine functions.
Application: Functional assays
Figure 1. Dose-dependent stimulation from arrestin recruitment upon treatment with ligand, measured with MULTISCREENTM β-Arrestin Assay Kit (Multispan MSBA01). Figure 2. Dose-dependent stimulation of intracellular cAMP level upon treatment with ligand, measured with MULTISCREENTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01). Figure 3. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Kobilka et al. (1987) cDNA for the human beta 2-adrenergic receptor: a protein with multiple membrane-spanning domains and encoded by a gene whose chromosomal location is shared with that of the receptor for platelet-derived growth factor. Proc Natl Acad Sci USA 84:46-50.
Frielle et al. (1989) Properties of the beta 1- and beta 2-adrenergic receptor subtypes revealed by molecular cloning. Clin Chem 35:721-725.