MULTISCREEN™ β-ARRESTIN2 STABLE CELL LINE
HUMAN RECOMBINANT CB2 RECEPTOR
Catalog Number: CA1230BA2-1
Lot Number: CA1230BA2-1-082321
Quantity: 1 vial (2 x 106) frozen cells
Freeze Medium: CellBanker2
Host cell: CHO-K1 β-Arrestin2
Transfection: Expression vector containing full-length human CNR2 cDNA (GenBank Accession Number NM_001841) with FLAG tag sequence at N-terminus and ARRB2 cDNA (GenBank Accession Number NM_004313.3)
Recommended Storage: Liquid nitrogen upon receiving
Propagation Medium: DMEM/F12, 10% FBS, 1 μg/mL puromycin, 800 μg/mL G418
Stability: In progress
Background: Cannabinoid receptor 2 (CB2 or CNR2) is involved in cannabinoid- induced central nerve system effects. It could be a receptor for anandamide. The receptor is a potential therapeutic target in the treatment of various disease conditions, such as pain, multiple sclerosis, vascular disease, Parkinson’s disease, and other central nerve system disorders.
Application: Functional assays
Figure 1. Dose-dependent stimulation from arrestin recruitment upon treatment with ligand, measured with MultiscreenTM β-Arrestin Assay Kit (Multispan MSBA01). Figure 2. Dose-dependent inhibition of forskolin-stimulated intracellular cAMP level upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01). Figure 3. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Mendizabal and Adler-Graschinsky (2003) Cannabinoid system as a potential target for drug development in the treatment of cardiovascular disease. Curr Vasc Pharmacol 1:301-313.
Gerard et al. (1990) Nucleotide sequence of a human cannabinoid receptor cDNA. Nucleic Acids Res 18:7142.