MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT β3 ADRENERGIC RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human ADRB3 cDNA (GenBank Accession Number NM_000025.1) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin
Stable in culture for minimum of two months
Background: The ß3-adrenoceptor is also known as the atypical beta- adrenoceptor. It was considered as a potential anti-obesity target by increasing energy expenditure and lipolysis in brown and white adipose tissues of rodents. The functional receptor has been demonstrated in a variety of other tissues including blood vessel, gastrointestinal tract, muscle, and retinal endothelial cells, and its agonist evokes relaxation in some of these tissues such as gall bladder, stomach, small intestine, prostate, colon and bladder.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Dose-dependent increase of intracellular cAMP level upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01). Figure 3. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Emorine et al. (1989) Molecular characterization of the human beta 3-adrenergic receptor. Science 245:1118-1121.
Fujimura et al. (1999) Expression and possible functional role of the beta3- adrenoceptor in human and rat detrusor muscle. J Urol. 161:680-685.