MULTISCREEN™ STABLE CELL LINES

HUMAN RECOMBINANT β1 ADRENERGIC RECEPTOR

Product Information

Catalog Number:
C1437-1

Lot Number:
C1437-1-092309

Quantity:
1 vial (2 x 106) frozen cells

Freeze Medium:
Sigma Freezing Medium (C-6164)

Host cell:
CHO-K1

Transfection:
Expression vector containing full-length human ADRB1 cDNA (GenBank Accession Number AF169007.1) with FLAG tag sequence at N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM/F12, 10% FBS, 800 μg/mL G418

Stability:
Stable in culture for minimum of two months

Data Sheet

Background: Norepinephrine is implicated in a wide range of physiological processes through activation of nine different G-protein-coupled receptors (α1a, α1b, α1d, α2a, α2b, α2c, ß1, ß2, ß3). The human ß1-adrenergic receptor is a 477–amino acid protein found in various heart and brain tissues. ß1 has an important role in the contractile action of valves in cardiac and digestive systems.

Application: Functional assays

Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Dose- dependent increase of intracellular cAMP level upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01). Figure 3. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti- FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.

References:

Oostendorp et al. (2000) Contribution of beta-adrenoceptor subtypes to relaxtion of colon and oesophagus and pacemaker activity of ureter in wildtype and beta(3)-adrenoceptor knockout mice. Br J Pharmacol 130:747-758.

Sato et al. (1996) Molecular characterization of pharmacological properties of T-0509 for beta-adrenoceptors. Eur J Pharmacol 315:363-367.

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