MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT α1b ADRENERGIC RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human ADRA1B cDNA (GenBank accession number NM_000679) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM/F12, 10% FBS, 10 μg/mL puromycin
Stable in culture for minimum of two months
Background: Norepinephrine is implicated in a wide range of physiological processes through activation of nine different G-protein-coupled receptors (α1a, α1b, α1d, α2a, α2b, α2c, ß1, ß2, ß3). The α1B-adrenoceptor expresses in brain, heart, kidney, and artery of various mammalian species and mediate diverse effects on cardiac functions, muscle contractions and cell growth. The knockout mouse models lacking the α1b-adrenergic receptor has highlighted the potential implication of this receptor subtype in variety of functions including the regulation of blood pressure, glucose homeostasis, reproduction, and the rewarding response to drugs of abuse. In vitro and in vivo studies also indicate that the α1b gene can function as an oncogene inducing neoplastic transformation.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Ramarao et al. (1992) Genomic organization and expression of the human alpha 1B- adrenergic receptor. J Biol Chem 267:21936-21945.
Allen et al. (1992) G protein-coupled receptor genes as protooncogenes: constitutively activating mutation of the alpha 1B-adrenergic receptor enhances mitogenesis and tumorigenicity. Proc Natl Acad Sci USA 88:11354-11358.
Weinberg et al. (1994) Cloning, expression and characterization of human alpha adrenergic receptors alpha 1a, alpha 1b and alpha 1c. Biochem. Biophys. Res. Commun. 201:1296-1304.