MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT α1a ADRENERGIC RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human ADRA1A cDNA (GenBank accession number NM_000680.2) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM/F12, 10% FBS, 10 μg/mL puromycin
Stable in culture for minimum of two months
Background: Norepinephrine is implicated in a wide range of physiological processes through activation of nine different G-protein-coupled receptors (α1a, α1b, α1d, α2a, α2b, α2c, ß1, ß2, ß3). The α1a-adrenoceptor is highly expressed in human vasculature. As with the other α1-ARs, α1a is used by the sympathetic nervous system to regulate systemic arterial blood pressure and blood flow. The α1-ARs also play a major role in cardiac and vascular smooth muscle cells. The knockout mouse models lacking the α1a-adrenergic receptors have highlighted the potential implications of this receptor subtype in variety of functions including the regulation of renal artery contractions, smooth muscle contractions, and vasoconstriction.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Chalothorn et al. (2002) Differences in the cellular localization and agonist-mediated internalization properties of the α1-adrenoreceptor subtypes. Mol Pharmacol 61(5):1008-1016.
Weinberg et al. (1994) Cloning, expression and characterization of human alpha adrenergic receptors alpha 1a, alpha 1b and alpha 1c. Biochem. Biophys. Res. Commun. 201:1296-1304.
Hague et al. (2003) a1-Adrenergic receptor subtypes: non-identical triplets with different dancing partners? Life Sciences 74:411-418.