MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT GPR97 RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human GPR97 cDNA (GenBank accession Number BC064508) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DME/F12, 10% FBS, 10 µg/mL puromycin
Background: Human G-protein coupled receptor 97 (ADGRG3), a member of the adhesion GPCR family, is a recently deorphanzied receptor for beclomethasone dipropionate. GPR97 is expressed in immune cells, specifically thymocytes and pre-B cells. Current research studies on knockout mice demonstrate the importance of GPR97 in B-cell development and its potential as a therapeutic target for the treatment of immunological disorders.
Application: Functional assays
Figure 1. Dose-dependent inhibition of forskolin-stimulated intracellular cAMP level upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Fredriksson R, Lagerström MC, Höglund PJ, Schiöth HB (Nov 2002). “Novel human G protein-coupled receptors with long N-terminals containing GPS domains and Ser/Thr-rich regions”. FEBS Letters 531 (3): 407–14.
Stacey M, Yona S (2011).Adhesion-GPCRs: Structure to Function (Advances in Experimental Medicine and Biology). Berlin: Springer.
Wang, J., Zhang, L., Zhang, H., Shen, C., Lu, S., Kuang, Y., … Wang, Z. (2013). Gpr97 is essential for the follicular versus marginal zone B-lymphocyte fate decision. Cell Death & Disease, 4(10), e853–. doi:10.1038/cddis.2013.346.