MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT GPR110 RECEPTOR
1 vial (2 x 106) frozen cells
Expression vector containing full-length human GPR110 cDNA (GenBank accession number NM_153840.2) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin
Background: GPR110 is an orphan G protein-coupled receptor. In a retroviral insertion mutagenesis screen in mouse, GPR110 has been identified as an oncogene. GPR110 has also been demonstrated as the synaptamide receptor, mediating synaptamide-induced bioactivity in a cAMP-dependent manner.
Application: Functional assays
Figure 1. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Ji-Won Lee1, et al. (2016) Orphan GPR110 (ADGRF1) targeted by N- docosahexaenoylethanolamine in development of neurons and cognitive function. NATURE COMMUNICATIONS. 7:13123. DOI: 10.1038/ncomms13123.
Amy Lum et al. (2010) Orphan receptor GPR110, an oncogene overexpressed in lung and prostate cancer. BMC Cancer, 2010, 10:40
Hannah M. Stovekena et al. (2003) Adhesion G protein-coupled receptors are activated by exposure of a cryptic tethered agonist. PNAS. 6194–6199 May 12, 2015. vol. 112 no. 19