MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT GPBAR1 RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human GPBAR1 cDNA (GenBank Accession Number NM_170699) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM/F12, 10% FBS, 10 μg/mL puromycin
Stable after minimum two months continuous growth
Background: The G protein-coupled bile acid receptor GPBAR1 (or GPR131) mediates bile acids-induced rapid elevation of intracellular cAMP levels. It is implicated in the suppression of macrophage functions and regulation of energy homeostasis by bile acids.
Application: Functional assays
Figure 1. Dose-dependent increase of intracellular cAMP upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Maruyama et al. (2002) Identification of membrane-type receptor for bile acids (M-BAR). Biochem Biophys Res Commun 298:714-719.
Katsuma et al. (2005) Bile acids promote glucagon-like peptide-1secretion through TGR5 in a murine enteroendocrine cell line STC-1. Biochem Biophys Res Commun 329:386-390.