MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT NPSR1 RECEPTOR (VARIANT 2)
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Full-length human NPSR1 variant 2 cDNA (GenBank Accession Number NM_207173)
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 100 µg/mL hygromycin
Stable after minimum of 2 months continuous growth
Background: Neuropeptide S (NPS) receptor 1 is also known as GPR154 or G protein-coupled receptor for asthma susceptibility (GPRA). NPS receptor is widely expressed in the brain, with highest levels found in hypothalamus, amygdala, endopiriform nucleus, cortex, subiculum and nuclei of the thalamic midline. Central administration of NPS promotes behavioral arousal and suppresses all stages of sleep in rodents. Furthermore, NPS was found to produce anxiolytic-like effects in a battery of four different tests that measure behavioral responses of rodents to novelty or stress.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Dose-dependent accumulation of intracellular IP1 upon treatment with ligand, measured with IP-one Tb kit (Cisbio 62IPAPEC).
Xu et al. (2004) Neuropeptide S: a neuropeptide promoting arousal and anxiolytic-like effects. Neuron 43:487-497.
Laitinen et al. (2004) Characterization of a common susceptibility locus for asthma-related traits. Science 304:300-304.