MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT C3A RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human C3AR1 cDNA (GenBank Accession Number NM_004054.2) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin
Stable after minimum of 2 months continuous growth
Background: C3a receptor (C3a anaphylatoxin chemotactic receptor) is a receptor for the chemotactic and inflammatory peptide anaphylatoxin C3a. The receptor is widely expressed in many hematopoietic cell lines, throughout the CNS and other tissues, and the expression level in glial cells increases during CNS inflammation. It stimulates chemotaxis, granule enzyme release and superoxide anion production, mediates the pathogenesis of demyelinating disease by directly or indirectly chemoattracting encephalitogenic cells to the CNS. Administration of small molecule inhibitors of C3a receptor inhibits airway responses.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Boos et al. (2004) Deletion of the complement anaphylatoxin C3a receptor attenuates, whereas ectopic expression of C3a in the brain exacerbates, experimental autoimmune encephalomyelitis. J Immunol 173:4708-14.
Ali and Panettieri, Jr. (2005) Anaphylatoxin C3a receptors in asthma. Respir Res 6:19.