MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT BB1 RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human NMBR cDNA (GenBank Accession Number NM_002511.1) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin
Stable after minimum of two months continuous growth
Background: The human BB1 receptor (or Neuromedin B receptor NMBR) is a receptor for neuromedin-B (NMB), which is a mammalian bombesin-like peptide distributed widely in the central nervous system. The BB1 pathway is involved in the regulation of a wide variety of behaviors, such as spontaneous activity, feeding and anxiety-related behavior. A study using BB1-deficient mice suggested that dysfunction in the BB1 pathway may constitute one of the risk factors of stress vulnerability.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Dose-dependent accumulation of intracellular IP1 upon treatment with ligand, measured with IP-one Tb kit (Cisbio 62IPAPEC). Figure 3. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Benya et al. (1995) Expression and characterization of cloned human bombesin receptors. Mol Pharmacol 47:10-20.
Moody et al. (2000) Nonpeptide neuromedin B receptor antagonists inhibit the proliferation of C6 cells. Eur J Pharmacol 409:133-142.
Yamada et al. (2002) Restraint stress impaired maternal behavior in female mice lacking the neuromedin B receptor (NMB-R) gene. Neurosci Lett 330:163-166.