MULTISCREEN™ STABLE CELL LINES

HUMAN RECOMBINANT B1 RECEPTOR

Product Information

Catalog Number:
C1198a

Lot Number:
C1198a-061510

Quantity:
1 vial (2 x 106) frozen cells

Freeze Medium:
Sigma Freezing Medium (C-6164)

Host cell:
HEK293T

Transfection:
Full-length Human BDKRB1 cDNA (GenBank Accession Number NM_000710) with FLAG-tag sequence at the N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin

Stability:
Stable in culture for minimum of two months

Data Sheet

Background: Bradykinin B1 is a receptor for bradykinin and mediates responses to pathophysiological conditions such as inflammation, trauma, burns, shock, and allergy. Receptors for B1 are normally absent, but become highly upregulated after inflammatory stimuli. B1 receptor agonists may have important clinical value in the treatment of chronic pain and inflammatory disorders.

Application: Functional assays

Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.

References:

Bastian et al. (1997) Stable expression of human kinin B1 receptor in 293 cells: pharmacological and functional characterization. Br J Pharmacol 122:393-399.

Calixto et al. (2004) Kinin B1 receptors: Key G-protein-coupled receptors and their role in inflammatory and painful processes. Br J Pharmacol 143:803-818.

Menke et al. (1994) Expression cloning of a human B1 bradykinin receptor. J Biol Chem 269:21583-21586.

FOR RESEARCH USE ONLY.
All rights reserved. No part of this document may be reproduced in any form without prior permission in writing.

26219 Eden Landing Road Hayward, CA 94545 | (510) 887-0817 | info@multispaninc.com