MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT B1 RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Full-length Human BDKRB1 cDNA (GenBank Accession Number NM_000710) with FLAG-tag sequence at the N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin
Stable in culture for minimum of two months
Background: Bradykinin B1 is a receptor for bradykinin and mediates responses to pathophysiological conditions such as inflammation, trauma, burns, shock, and allergy. Receptors for B1 are normally absent, but become highly upregulated after inflammatory stimuli. B1 receptor agonists may have important clinical value in the treatment of chronic pain and inflammatory disorders.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Bastian et al. (1997) Stable expression of human kinin B1 receptor in 293 cells: pharmacological and functional characterization. Br J Pharmacol 122:393-399.
Calixto et al. (2004) Kinin B1 receptors: Key G-protein-coupled receptors and their role in inflammatory and painful processes. Br J Pharmacol 143:803-818.
Menke et al. (1994) Expression cloning of a human B1 bradykinin receptor. J Biol Chem 269:21583-21586.