MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT CXCR3 RECEPTOR
1 vial (2 x 106) frozen cells
Cell Banker 2 (Amsbio 11891)
Expression vector containing full-length human CXCR3 cDNA (GenBank Accession Number: NM_001504) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin
Stable in culture for minimum of two months
Background: CXCR3 (chemokine receptor 3) is a receptor for SCYB9/MIG, SCYB10/INP10 and SCYB11/ITAC. The chemokines that target CXCR3 in concert with Th1 cytokine appear to play a beneficial role to atopic disorders including a range of conditions such as allergic asthma, -rhinitis, -conjunctivitis, -dermatitis, food and drug allergies and anaphylaxis.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Dose-dependent inhibition of forskolin-stimulated intracellular cAMP accumulation upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01). Figure 3. Receptor Expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
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Arimilli, S., W. Ferlin, et al. (2000). “Chemokines in autoimmune diseases.” Immunol Rev 177: 43-51.