MULTISCREEN™ STABLE CELL LINES

RECOMBINANT SMOOTHENED

Product Information

Catalog Number:
H1442

Lot Number:
H1442-080712

Quantity:
1 vial (2 x 106) frozen cells

Freeze Medium:
Sigma Freezing Medium (C-6164)

Host cell:
HEK293T

Transfection:
Expression vector containing full-length human SMO cDNA (GenBank Accession Number: NM_005631.3) with FLAG tag sequence at N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin

Stability:
In progress

Data Sheet

Background: The human smoothened homolog SMO strongly resembles that of the frizzled family of proteins, the receptors of the Wnt signaling pathway, which share several features with the Hedgehog pathway. Most often SMO functions during embryonic development, in processes such as digit patterning in the chick limb bud and left–right asymmetry of vertebrate embryos. In addition, SMO function is fundamental for the maintenance of tissue homeostasis in adults, and deregulated SMO signaling is implicated in tumorogenesis.

Application: Binding assays

Figure 1. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody

Reference:

Ruiz-Gómez et al. (2007) The cell biology of Smo signaling and its relationships with GPCRs. Biochim Biophys Acta 1768:901-912.

FOR RESEARCH USE ONLY.
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