MULTISCREEN™ STABLE CELL LINES

HUMAN RECOMBINANT GPR55 RECEPTOR

Product Information

Catalog Number:
H1113

Lot Number:
H1113-021210

Quantity:
1 vial (2 x 106) frozen cells

Freeze Medium:
Sigma Freezing Medium (C-6164)

Host cell:
HEK293T

Transfection:
Expression vector containing full-length human GPR55 cDNA (GenBank Accession Number NM_005683.3) with FLAG tag sequence at N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin

Stability:
In progress

Data Sheet

Background: GPR55 is a putative cannabinoid receptor. Its gene was mapped to chromosome 2q37, using fluorescence in situ hybridization (FISH), and its mRNA transcripts have been detected in the caudate nucleus and putamen. Recently, GPR55 was identified as a receptor for the bioactive lipid lysophosphatidylinositol (LPI).

Application: Functional assays

Figure legend. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor- expressing cells.

References:

Sawzdargo et al. (1999). Identification and cloning of three novel human G protein- coupled receptor genes GPR52, PsiGPR53 and GPR55: GPR55 is extensively expressed in human brain. Brain Res Mol Brain Res 64:193-198.

Oka et al. (2007) Identification of GPR55 as a lysophosphatidylinositol receptor. Biochem Biophys Res Commun 362:928–934.

FOR RESEARCH USE ONLY.
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