MULTISCREEN™ STABLE CELL LINES
RAT RECOMBINANT GPR43 RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length rat FFAR2 cDNA (GenBank Accession Number NM_001005877.1) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin
Background: GPR43, or free fatty acid receptor 2 (FFAR2), encodes a deduced 330- amino acid protein with 7 transmembrane domains. GPR43 is expressed by enteroendocrine cells expressing peptide YY and mucosal mast cells in the rat intestine. The receptor binding of short-chain fatty acids potentially provides an intestine molecular link between diet, gastrointestinal bacterial metabolism, and immune and inflammatory responses.
Figure 1. Dose-dependent calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Dose- dependent inhibition of forskolin-stimulated intracellular cAMP level upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01). Figure 3. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Karaki et al. (2006) Short-chain fatty acid receptor, GPR43, is expressed by enteroendocrine cells and mucosal mast cells in rat intestine. Cell Tissue Res324(3):353-60.
Sawzdargo et al. (1997) Cluster of four novel human G protein-coupled receptor genes occurring in close proximity to CD22 gene on chromosome 19q13.1. Biochem Biophys Res Commun 239:543-547.