MULTISCREEN™ STABLE CELL LINES
MOUSE RECOMBINANT 5-HT2B RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Full-length Mouse HTR2B cDNA (GenBank Accession Number NM_008311) with FLAG-tag sequence at the N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin
Stable in culture for a minimum of two months
Background: 5-HT2B (5-hydroxytryptamine receptor 2B) is a receptor for serotonin. It is expressed in many peripheral and central nervous system tissues, including stomach fundus, liver kidney, muscle, intestine and brain. 5-HT2B receptors are responsible for many cardiovascular and central nervous system functions, such as blood vessel contraction, platelet shape changes, neuronal sensitization to tactile stimuli, and mediation of the hallucinogenic effects of phenylisopropylamin hallucinogens. It has also been shown to be required for heart development.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Bonhaus et al. (1995) The pharmacology and distribution of human 5- hydroxytryptamine 2B (5-HT2B) receptor gene products: comparison with 5-HT2A and 5-HT2C receptors. Br J Pharmacol 115:622-628.
Nebegil et al. (1992) Serotonin 2B receptor is required for heart development. Proc Natl Acad Sci USA 97:9508-9513.
Porter et al. (1999) Functional characterization of agonists at recombinant human 5- HT2A, 5-HT2B, and 5-HT2C receptors in CHO-K1 cells. Br J Pharmacol 128:13-20.