MULTISCREEN™ STABLE CELL LINES

MOUSE RECOMBINANT EP2 RECEPTOR

Product Information

Catalog Number:
Cm1202

Lot Number:
Cm1202-101117

Quantity:
1 vial (2 x 106) frozen cells

Freeze Medium:
Cell Banker 2 (Amsbio 11891)

Host cell:
HEK293T

Transfection:
Expression vector containing full-length mouse EP2 cDNA (GenBank Accession Number NM_008964.4) with FLAG tag sequence at N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin

Stability:
Stable in culture for minimum of two months

Data Sheet

Background: The human prostaglandin E2 (PGE2) receptor EP2 (PTGER2) is abundantly expressed in various tissues including the corneal epithelium of the eye, spinal cord, forebrain, articular cartilage, and kidney. EP2 plays important roles in bronchodilation, dilation of arterioles and venules, blood pressure regulation, smooth muscle relaxation, and bone formation. Modification of PGE2-EP2 receptor signaling may provide a new therapeutic strategy for renal regulation and blood pressure illnesses, as well as bone disease such as osteoarthritis.

Application: Functional assays

Figure 1. Dose-dependent accumulation of intracellular cAMP upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cellsthick line: receptor-expressing cells.

References:

Morath et al. (1999) Immunolocalization of the four prostaglandin E2 receptor proteins EP1, EP2, EP3, and EP4 in human kidney. J Am Soc Nephrol 10:1851-1860.

Zhang et al. (2000) Characterization of murine vasopressor and vasodepressor prostaglandin E(2) receptors. Hypertension 35:1129-1134.

Li X et al. (2009) Prostaglandin E(2) and its cognate EP receptors control human adult articular cartilage homeostasis and are linked to the pathophysiology of osteoarthritis. Arthritis Rheum 60:513-523.

FOR RESEARCH USE ONLY.
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