MULTISCREEN™ STABLE CELL LINES

MOUSE RECOMBINANT GPR43 RECEPTOR

Product Information

Catalog Number:
Cm1104

Lot Number:
Cm1104-122713

Quantity:
1 vial (2 x 106) frozen cells

Freeze Medium:
Sigma Freezing Medium (C-6164)

Host cell:
HEK293T

Transfection:
Expression vector containing full-length mouse FFAR2 cDNA (GenBank Accession Number NM_146187.4) with FLAG tag sequence at N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin

Stability:
In progress

Data Sheet

Background: GPR43, or free fatty acid receptor 2 (FFAR2), encodes a deduced 330- amino acid protein with 7 transmembrane domains. GPR43 is expressed by white adipose tissue and immune tissue of adult mice. The receptor binding of short-chain fatty acids potentially provides an intestine molecular link between diet, gastrointestinal bacterial metabolism, and immune and inflammatory responses.

Application: Functional assays

Figure 1. Dose-dependent calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Dose- dependent inhibition of forskolinstimulated intracellular cAMP accumulation upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01). Figure 3. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.

References:

Kimura, I. et al. The gut microbiota suppresses insulin-mediated fat accumulation via the short-chain fatty acid receptor GPR43. Nat. Commun. 4:1829 doi: 10.1038/ncomms2852 (2013).
 
Sawzdargo et al. (1997) Cluster of four novel human G protein-coupled receptor genes occurring in close proximity to CD22 gene on chromosome 19q13.1. Biochem Biophys Res Commun 239:543-547.

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