MULTISCREEN™ STABLE CELL LINES

MOUSE RECOMBINANT S1P2 RECEPTOR

Product Information

Catalog Number:
Cm1051-1

Lot Number:
Cm1051-1-121018

Quantity:
1 vial (2 x 106) frozen cells

Freeze Medium:
Cellbanker 2 (Amsbio 11891)

Host cell:
CHO-K1

Transfection:
Expression vector containing full-length mouse S1P2 cDNA (Genbank Accession Number: NM_010333.4) with FLAG tag sequence at N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM/F12, 10% FBS, 10 μg/mL puromycin

Stability:
In progress

Data Sheet

Background: DG-5 (Endothelial Differentiation Gene) is a member of G protein coupled sphingosine-1-phosphate receptor family, which includes S1P1 (EDG-1), S1P2 (EDG-5/H218/AGR16), S1P3 (EDG-3), S1P4 (EDG-6), and S1P5 (EDG- 8/NRG-1). Sphingosine-1-phosphate (SPP) is a bioactive lipid produced from the metabolism of sphingomyelin. It is an important constituent of serum and regulates cell growth, survival, migration, differentiation and gene expression via its interaction with the S1P family of G-protein coupled receptors. EDG-5 couple to a variety of G proteins i.e. Gi, Gq, G12, and G13 to activate extracellular signal-regulated kinase and mobilize Ca2+ and activate Elk-1- and serum-response factor (SRF)-driven gene transcription. Recent data suggest that EDG-5 also regulates Rho/Rho kinase pathway to inhibit tumor cell migration

Application: Functional assays

Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.

References:

IM et al. (2000) Characterization of a novel phingosine 1-phosphate receptor, Edg-8. J Biol Chem 275:14281-14286.

FOR RESEARCH USE ONLY.
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