MULTISCREEN™ STABLE CELL LINES
MOUSE RECOMBINANT H1 RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length mouse HRH1 cDNA (GenBank Accession Number NM_008285) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin
Stable in culture for minimum of two months
Background: Histamine H1 receptor (H1 or HRH1) is a subclass of histamine receptors. It mediates the contraction of smooth muscles, increase in capillary permeability due to contraction of terminal venules, and catecholamine release from adrenal medulla, and neurotransmission in the central nervous system. Antihistamines including H1 receptor antagonists are among the most frequently used pharmacologic agents because of a high incident rate of upper respiratory allergies. Clinical and laboratory evidence on histamine and its actions suggests that it has a pathophysiologic role in asthma. These findings have renewed interest in the potential therapeutic role of H1-receptor antagonists, such as, fexofenadine, which has been investigated as potential therapeutic targets for this disease.
Application: Functional assays
Figure 1. Dose-dependent calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Cuss (1999) Beyond the histamine receptor: effect of antihistamines on mast cells. Clin Exp Allergy 29 Suppl 3:54-59
Gelfand (2002) Role of histamine in the pathophysiology of asthma: immunomodulatory and anti-inflammatory activities of H1-receptor antagonists. Am J Med 113 Suppl 9A:2S-7S.