MULTISCREEN™ STABLE CELL LINES

DOG RECOMBINANT GPR35 RECEPTOR

Product Information

Catalog Number:
CGd1096-1

Lot Number:
CGd1096-1-091217

Quantity:
1 vial (2 x 106) frozen cells

Freeze Medium:
Cellbanker 2 (Amsbio 11891)

Host cell:
CHO-K1 Gα16

Transfection:
Expression vector containing full-length dog GPR35 cDNA (GenBank Accession Number: ENSCAFT00000036760) with FLAG tag sequence at N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM/F12, 10% FBS, 10 µg/mL puromycin, 800 μg/mL G418

Stability:
In progress

Data Sheet

Background: GPR35 is an orphan receptor and expressed in various tissues including stomach, gastrointestinal tissues, and several types of immune cells. Up- regulation of GPR35 has been found in human mast cells upon stimulation with IgE antibodies, human macrophages treated with the environmental contaminant polycyclic aromatic hydrocarbon benzo[a]pyrene, failing heart cells, and gastric cancer cells. Known agonists of the orphan receptor GPR35 are kynurenic acid, zaprinast, 5-nitro-2-(3-phenylproplyamino) benzoic acid, and lysophosphatidic acids.

Application: Functional assays

Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Dose-dependent stimulation of pERK level upon treatment with ligand, monitored with FlexStation (Cisbio 64AERPEG).

References:

Wang JH et al. (2006) Kynurenic Acid as a Ligand for Orphan G Protein-coupled Receptor GPR35. J Biol Chem 281:22021-22028.

MacKenzie1, AE, et al. (2011) GPR35 as a novel therapeutic target. Frontiers in Endocrinology, 2:1-10.

Zhao PW, et al. (2010) Targeting of the Orphan Receptor GPR35 by Pamoic Acid: A Potent Activator of Extracellular Signal-Regulated Kinase and beta-Arrestin2 with Antinociceptive Activity. Mol Pharm 78:560–568.

FOR RESEARCH USE ONLY.
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