MULTISCREEN™ STABLE CELL LINES

DOG RECOMBINANT CCR9 RECEPTOR

Product Information

Catalog Number:
CGd1017

Lot Number:
CGd1017-111213

Quantity:
1 vial (2 x 106) frozen cells

Freeze Medium:
Sigma Freezing Medium (C-6164)

Host cell:
HEK293T Gαqi5

Transfection:
Expression vector containing full-length human CCR9 cDNA (GenBank Accession Number: NM_001284476) with FLAG tag sequence at N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM, 10% FBS,1 g/mL puromycin, 250 µg/mL hygromycin

Stability:
Stable in culture for minimum of two months

Data Sheet

Background: CCR9 (C-C chemokine receptor type 9) is a thymus-specific receptor for chemokine CCL25 (SCYA25 or TECK). It is an alternative co-receptor with CD4 for HIV-1 infection. Activation of the receptor may lead to potent cFLIP(L)- independent resistance to cycloheximide-induced apoptosis and modest resistance to Fas-mediated apoptosis possibly via activation of multiple signaling components involving Akt and glycogen synthase kinase 3β. Thus, dissection of signaling components involved in the CCR9-mediated antiapoptosis could be a framework for cell survival mechanisms and may provide options for therapeutic interventions.

Application: Functional assays

Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.

References:

Youn et al. (2002) Role of the CC chemokine receptor 9/TECK interaction in apoptosis. Apoptosis 7:271-276.

Zaballos et al. (1999) Cutting edge: identification of the orphan chemokine receptor GPR-9-6 as CCR9, the receptor for the chemokine TECK. J Immunol 162(10):5671- 5.

FOR RESEARCH USE ONLY.
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