MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT NPFF2 RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human NPFF1 cDNA (GenBank Accession Number NM_004885.1) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin
Stable in culture for minimum of two months
Background: Neuropeptide FF receptor 2 (NPFF2) belongs to the rhodopsin family of G protein-coupled receptors and found across the central nervous system. It is believed to regulate endogenous opioid systems in the brain and spinal cord. The neuropeptide is considered a prospective target to decrease negative effects of chronic opioid use.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).
Bonini, James et al. “Identification and Characterization of Two G Protein-coupled Receptors for Neuropeptide FF.” The Journal of Biological Chemistry. Vol. 275, No. 50, pp. 39324-39331. Dec 2000
Elshourbagy, Nabil et al. “Receptor for the Pain Modulatory Neuropeptides FF and AF is an Orphan G Protein-coupled Receptor.” The Journal of Biological Chemistry. Vol. 275, No. 34, pp. 25965-25971. Aug 2000
Mollereau, Catherine et al. “Agonist and Antagonist Activities on Human NPFF2 Receptors of the NPY Ligands GR231118 and BIBP3226.” British Journal of Pharmacology 133.1 (2001): 1–
4. PMC. Web. 5 Mar. 2015.
Moulédous, Lionel et al. “Opposite control of body temperature by NPFF1 and NPFF2 receptors in mice.” Neuropeptides 44.5: 453 – 456.