MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT SST5 RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Full-length Human SSTR5 cDNA (GenBank Accession Number NM_001053) with FLAG-tag sequence at the N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM/F12, 10% FBS, 800 μg/mL G418, 10 μg/mL puromycin
Stable in culture for minimum of two months
Background: Somatostatin receptors are activated by somatostatin secreted from nerve and endocrine cells. The Somatostatin Receptors (SSTRs) are expressed in a tissue-specific manner and involved in the regulation of secretion of insulin, glucagon and growth hormone as well as cell growth induced by neuronal excitation in both the central and peripheral nervous systems. Aberrant expression of somatostatin receptors is known in a large number of human tumors. The human medullary thyroid carcinoma cell line TT expresses all SSTR subtypes. SST5 is expressed in adult pituitary gland, heart, small intestine, adrenal gland, cerebellum and fetal hypothalamus. Expression has not been detected in fetal or adult kidney, liver, pancreas, uterus, spleen, lung, thyroid or ovary. SST5 is a candidate gene for bipolar affective disorder (BPAD) as well as for other neuropsychiatric disorders. Expression of SST2 and SST5 may be important in the growth inhibitory effect of somatostatin in human pancreatic cancer.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Ardjomand et al. (2003) Expression of somatostatin receptors in uveal melanomas. Invest Ophthal Vis Sci 44:980-987.
Ballare et al. (2001) Mutation of somatostatin receptor type 5 in an acromegalic patient resistant to somatostatin analog treatment. J Clin Endocr Metab 86:3809- 3814.