MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT SST2 RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human SSTR2 cDNA (GenBank Accession Number AY236542) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM/F12, 10% FBS, 800 μg/mL G418, 10 μg/mL puromycin
Stable after minimum of two months continuous growth
Background: Somatostatin receptors are activated by somatostatin secreted from nerve and endocrine cells. The Somatostatin Receptors (SSTRs) are expressed in a tissue-specific manner and involved in the regulation of secretion of insulin, glucagon and growth hormone as well as cell growth induced by neuronal excitation in both the central and peripheral nervous systems. Aberrent expression of somatostatin receptors is known in a large number of human tumours. SSTR2 is expressed in highest levels in the stomach, jejunum, cerebrum, thyroid and kidney. Its gene expression is lost in 90% of human pancreatic adenocarcinomas while SSTR2 expression in neuroblastoma correlates positively to overall and event-free survival. Expression of SSTR-2 and SSTR-5 may be important in the growth inhibitory effect of somatostatin in human pancreatic cancer.
Application: Functional assays
Figure 1. Dose-dependent stimulation of intercellular calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Ardjomand et al. (2003) Expression of somatostatin receptors in uveal melanomas. Invest Ophthal Vis Sci 44:980-987.
Bertherat et al. (2003) Somatostatin receptors 2 and 5 are the major somatostatin receptors in insulinomas: an in vivo and in vitro study. J Clin Endocr Metab 88:5353- 5360.