MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT SST1 RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human SSTR1 cDNA (GenBank Accession Number AY322536) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM/F12, 10% FBS, 800μg/mL G418, 10 μg/mL puromycin
Stable after minimum of two months continuous growth
Background: Somatostatin receptors are activated by somatostatin secreted from nerve and endocrine cells. The Somatostatin Receptors (SSTRs) are expressed in a tissue-specific manner and involved in the regulation of secretion of insulin, glucagon and growth hormone as well as cell growth induced by neuronal excitation in both the central and peripheral nervous systems. Aberrent expression of somatostatin receptors is known in a large number of human tumours. The human medullary thyroid carcinoma cell line TT expresses all SSTR subtypes. SSTR1 has been reported at highest levels in the jejunum and stomach. In addition, this receptor is expressed in adrenal, brain, liver, lung, eye, and pancreas. ESTs from SSTR1 have been isolated from normal placenta and colon cancer libraries. Normal T-cells expressed SSTR1 and SSTR5 while T-cell leukaemia lines do not. Selective activation of SSTR1 inhibits hormone secretion and cell viability in GH- secreting and PRL-secreting adenomas in vitro.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Ardjomand et al. (2003) Expression of somatostatin receptors in uveal melanomas. Invest Ophthal Vis Sci 44:980-987.
Bertherat et al. (2003) Somatostatin receptors 2 and 5 are the major somatostatin receptors in insulinomas: an in vivo and in vitro study. J Clin Endocr Metab 88:5353- 5360.