MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT NPY4 RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C- 6164)
Expression vector containing full-length human Y4 cDNA (GenBank Accession Number AY268432) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM-F12, 10% FBS, 10 μg/mL puromycin, 150 μg/mL hygromycin
Stable after minimum of two months continuous growth
Background: NPY4R (Y4) has been reported to be expressed at highest levels in brain, coronary artery and ileum, low levels in pancreas and kidney. It is also been detected in colon and small intestine and isolated from human colon and head/neck libraries. It is one of the receptors for neuropeptide Y and peptide YY. The rank order of affinity of this receptor for pancreatic polypeptides is PP, PP (2-36) and [Ile- 31, Gln-34] PP > [Pro-34] PYY > PYY and [Leu-31, Pro-34] NPY > NPY > PYY (3- 36) and NPY (2-36) > PP (13-36) > PP (31-36) > NPY free acid. Ligand: Neuropeptide Y, Peptide YY.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor- expressing cells.
Bard et al. (1995) Cloning and functional expression of a human Y4 subtype receptor for pancreatic polypeptide, neuropeptide Y, and peptide YY. J Biol Chem270:26762-26765.
Yan et al. (1996) Cloning and functional expression of cDNAs encoding human and rat pancreatic polypeptide receptors. Proc Natl Acad Sci USA 93:4661-4665.