MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT NPY2 RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human NPY2R cDNA (GenBank Accession Number NM_00091) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM-F12, 10% FBS, 10 μg/mL puromycin, 150 μg/mL hygromycin
Stable after two months continuous growth
Background: NPY2R (Y2) belongs to the family of G protein-coupled receptors. It is expressed at high levels in central nerve system, heart, ileum and colon. NPY2R shows high affinity for neuropeptide Y (NPY), peptide YY (PYY), and a fragment of NPY including amino acids 13 to 36. The rank order of affinity of this receptor for pancreatic polypeptides is PYY > NPY > PYY (3-36) > NPY (2-36) > [Il-31, Gln-34] PP > [Leu-31, Pro-34] NPY > PP, [Pro-34] PYY and NPY free acid. NPY exhibits a variety of potent central and peripheral effects including those on feeding, memory, blood pressure, and intestinal secretions. NPY2R has been shown to contribute to obesity.
Application: Functional assays
Figure 1. Dose-dependent calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Dose- dependent cAMP accumulation upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01). Figure 3. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Gerald et al. (1995) Expression cloning and pharmacological characterization of a human hippocampal neuropeptide Y/peptide YY Y2 receptor subtype. J Biol Chem 270:26758- 26761.
Lutz et al. (1997) Neuropeptide Y receptor genes on human chromosome 4q31-q32 map to conserved linkage groups on mouse chromosomes 3 and 8. Genomics 41:498-500.
Rose et al. (1995) Cloning and functional expression of a cDNA encoding a human type 2 neuropeptide Y receptor. J Biol Chem 270:22661-22664.