MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT NPY1 RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human NPY1R cDNA (GenBank Accession Number NM_000909) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM-F12, 10% FBS, 10 μg/mL puromycin, 150 μg/mL hygromycin
Stable after two months continuous growth
Background: NPY1R (Neuropeptide Y receptor type 1) is a receptor for neuropeptide Y that is the most abundant peptide present in the mammalian central and peripheral nervous system. Neuropeptide Y exhibits a variety of potent central and peripheral effects including those on feeding, memory, blood pressure, cardiac contractility and intestinal secretions. Neuropeptide Y effects are mediated by several different receptor subtypes, Y- 1, Y-1-like, Y-2, Y-3, Y-4 and Y-5. NPY1R is correlated with food feeding and subnanomolar antagonists of NPY1R have been discovered as potential anti-obesity agents. NPY1R is also involved in cardiovascular actions of exogenous neuropeptide Y and plays a role in maintaining basal blood pressure and heart rate in ischaemic heart failure.
Application: Functional assays
Figure 1. Dose-dependent calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Dose- dependent inhibition of forskolin-stimulated intracellular cAMP level upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01). Figure 3. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor- expressing cells.
Balasubramaniam (1997) Neuropeptide Y family of hormones: receptor subtypes and antagonists. Peptides 18:445-457.
Hammond (2001) Neuropeptide Y receptor antagonists. IDrugs 4:920-927.
Zhao et al. (1999) Renal and cardiovascular role of the neuropeptide YY1 receptor in ischaemic heart failure rats. J Pharm Pharmacol 51:1257-1265.