MULTISCREEN™ STABLE CELL LINES

HUMAN RECOMBINANT EBI2 RECEPTOR

Product Information

Catalog Number:
CG1242-1

Lot Number:
CG1242-1-042814

Quantity:
1 vial (2 x 106) frozen cells

Freeze Medium:
Sigma Freezing Medium (C-6164)

Host cell:
CHO-K1 Gαqi5

Transfection:
Expression vector containing full-length human EBI2 cDNA (GenBank Accession Number NM_004951) with FLAG tag sequence at N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM/F12, 10% FBS, 10 μg/ml puromycin, 250 μg/mL hygromycin

Stability:
Stable in culture for minimum of two months

Data Sheet

Background: Epstein-Barr virus-induced gene 2 (EBI2, also known as GPR183) is a GPCR required for humoral immune responses and polymorphisms have been associated with inflammatory autoimmune diseases. It is expressed in B-lymphocyte cell lines as well as lymphoid tissues but not T-lymphocyte cell lines or peripheral blood T-lymphocytes. 7Į,25-dihydroxycholesterol (7Į,25-OHC) and other oxysterols act as chemoattractants for immune cells expressing EBI2 by directing cell migration. Mice deficient in cholesterol 25- hydroxylase (CH25H, required for generation of 7Į,25-OHC) fail to generate EBI2 biological activity in vivo and shows that the EBI2-oxysterol signaling pathway plays an important role in the adaptive immune response.

Application: Functional assays

Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-MOR antibody. Thin line: Cells stained with secondary antibody only; Thick line: Cells stained with primary and secondary antibody.

References:

Chen et al. (1993) Molecular cloning and functional expression of a mu-opioid receptor from rat brain. Mol Pharmacol 44:8-12.

Contet et al. (2004) Mu opioid receptor: a gateway to drug addiction. Curr Opin Neurobiol 14:370-378.

Philippe et al. (2003) Anti-inflammatory properties of the mu opioid receptor support its use in the treatment of colon inflammation. J Clin Invest 111:1329-1338.

 

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