MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT EBI2 RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human EBI2 cDNA (GenBank Accession Number NM_004951) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM/F12, 10% FBS, 10 μg/ml puromycin, 250 μg/mL hygromycin
Stable in culture for minimum of two months
Background: Epstein-Barr virus-induced gene 2 (EBI2, also known as GPR183) is a GPCR required for humoral immune responses and polymorphisms have been associated with inflammatory autoimmune diseases. It is expressed in B-lymphocyte cell lines as well as lymphoid tissues but not T-lymphocyte cell lines or peripheral blood T-lymphocytes. 7Į,25-dihydroxycholesterol (7Į,25-OHC) and other oxysterols act as chemoattractants for immune cells expressing EBI2 by directing cell migration. Mice deficient in cholesterol 25- hydroxylase (CH25H, required for generation of 7Į,25-OHC) fail to generate EBI2 biological activity in vivo and shows that the EBI2-oxysterol signaling pathway plays an important role in the adaptive immune response.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-MOR antibody. Thin line: Cells stained with secondary antibody only; Thick line: Cells stained with primary and secondary antibody.
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