MULTISCREEN™ STABLE CELL LINES

HUMAN RECOMBINANT CMKLR1 RECEPTOR

Product Information

Catalog Number:
CG1147

Lot Number:
CG1147-022510

Quantity:
1 vial (2 x 106) frozen cells

Freeze Medium:
Sigma Freezing Medium (C-6164)

Host cell:
HEK293T Gαqi5

Transfection:
Expression vector

containing full-length human CMKLR1 cDNA (GenBank Accession Number: NM_004072) with FLAG tag sequence at N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM, 10% FBS, 1 µg/mL puromycin, 150 µg/mL hygromycin

Stability:
Stable after minimum of two months continuous growth

Data Sheet

Background: Chemokine receptor-like 1 (CMKLR1) (or ChemR23) is a receptor for TIG2 and Chemerin. ChemR23/Chemerin may play a key role in directing plasmacytoid dendritic cells trafficking. The receptor also acts as a coreceptor for several SIV strains (SIVMAC316, SIVMAC239, SIVMACL7E-FR and SIVSM62A), as well as a primary HIV-1 strain (92UG024-2).

Application: Functional assays

Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.

References:

Meder et al. (2003) Characterization of human circulating TIG2 as a ligand for the orphan receptor ChemR23. FEBS Lett 555:495-499.

Wittamer et al. (2004) The C-terminal nonapeptide of mature chemerin activates the chemerin receptor with low nanomolar potency. J Biol Chem 279:9956-9962.

Samson et al. (1998) ChemR23, a putative chemoattractant receptor, is expressed in monocyte-derived dendritic cells and macrophages and is a coreceptor for SIV and some primary HIV-1 strains. Eur J Immunol 28:1689-1700.

FOR RESEARCH USE ONLY.
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