MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT S1P2 RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human S1P2 cDNA (Genbank Accession Number: NM_004230) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM/F12, 10% FBS, 250 μg/mL hygromycin, 10 μg/mL puromycin
Stable after minimum of two months continuous growth
Background: EDG-5 (Endothelial Differentiation Gene) is a member of G protein coupled sphingosine-1-phosphate receptor family, which includes S1P1 (EDG-1), S1P2 (EDG-5/H218/AGR16), S1P3 (EDG-3), S1P4 (EDG-6), and S1P5 (EDG- 8/NRG-1). Sphingosine-1-phosphate (SPP) is a bioactive lipid produced from the metabolism of sphingomyelin. It is an important constituent of serum and regulates cell growth, survival, migration, differentiation and gene expression via its interaction with the S1P family of G-protein coupled receptors. EDG-5 couple to a variety of G proteins i.e. Gi, Gq, G12, and G13 to activate extracellular signal-regulated kinase and mobilize Ca2+ and activate Elk-1- and serum-response factor (SRF)-driven gene transcription. Recent data suggest that EDG-5 also regulates Rho/Rho kinase pathway to inhibit tumor cell migration
Application: Functional assays
Figure Legend. Dose response of intracellular calcium as monitored with FlexStation upon treatment with ligand.
IM et al. (2000) Characterization of a novel phingosine 1-phosphate receptor, Edg-8. J Biol Chem 275:14281-14286.