MULTISCREEN™ STABLE CELL LINES

HUMAN RECOMBINANT S1P3 RECEPTOR

Product Information

Catalog Number:
CG1049-1

Lot Number:
CG1049-1-092107

Quantity:
1 vial (2 x 106) frozen cells

Freeze Medium:
Sigma Freezing Medium (C-6164)

Host cell:
CHO-K1 Gαqi5

Transfection:
Expression vector containing full-length human S1P3 cDNA (Genbank Accession Number NM_005226.2) with FLAG tag sequence at N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM/F12, 10% FBS, 250 μg/mL hygromycin, 10 μg/mL puromycin

Stability:
Stable in culture for minimum of two months

Data Sheet

Background: S1P3 (sphingosine 1-phosphate receptor 3) or EDG3 (endothelial differentiation G-protein coupled receptor 3) is a receptor for the lysosphingolipid sphingosine 1-phosphate, a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. EDG receptors mediate pro- angiogenic activities, such as endothelial cell proliferation, chemotaxis, and vessel morphogenesis. S1P3, along with S1P1, is required for directing the organization of endothelial cells into capillary networks.

Application: Functional assays

Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.

References:

Takuwa et al. (2001) Subtype-specific, differential activities of the EDG family receptors for sphingosine-1-phosphate, a novel lysophospholipid mediator. Mol Cell Endocrinol 177:3-11.

FOR RESEARCH USE ONLY.
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