MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT CCR10 RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Full-length human CCR10 cDNA (GenBank accession Number NM_016602.2) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM/F12, 10% FBS, 10 μg/mL puromycin, 250 μg/mL hygromycin
Background: CCR10 (also known as GPR2) is selectively expressed by IgA- secreting cells including circulating IgA+ plasmablasts, as well as almost all IgA+ plasma cells in the salivary gland, small intestine, large intestine, appendix and tonsils. It is a receptor for CCL27/CTACK and CCL28/MEC. Expression of CCL27 in skin may play an important role in facilitating memory T cells that express CCR10 to enter into cutaneous sites during inflammation and in determining the metastatic destination of malignant melanoma.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Muller et al. (2001) Involvement of chemokine receptors in breast cancer metastasis. Nature 410:50-56.
Kunkel et al. (2003) CCR10 expression is a common feature of circulating and mucosal epithelial tissue IgA Ab-secreting cells. J Clin Invest 111:1001-1010.
Hudak et al. (2002) Immune surveillance and effector functions of CCR10(+) skin homing T cells. J Immunol 169:1189-1196.