MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT CCR9 RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human CCR9 cDNA (GenBank Accession Number NM_006641) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin, 250 μg/mL hygromycin
Stable for minimum two months of continuous growth.
Background: CCR9 (C-C chemokine receptor type 9) is a thymus-specific receptor for chemokine CCL25 (SCYA25 or TECK). It is an alternative co-receptor with CD4 for HIV-1 infection. Activation of the receptor may lead to potent cFLIP(L)- independent resistance to cycloheximide-induced apoptosis and modest resistance to Fas-mediated apoptosis possibly via activation of multiple signaling components involving Akt and glycogen synthase kinase 3β. Thus, dissection of signaling components involved in the CCR9-mediated antiapoptosis could be a framework for cell survival mechanisms and may provide options for therapeutic interventions.
Application: Functional assays
Figure legend: Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).
Youn et al. (2002) Role of the CC chemokine receptor 9/TECK interaction in apoptosis. Apoptosis 7:271-276.
Zaballos et al. (1999) Cutting edge: identification of the orphan chemokine receptor GPR-9-6 as CCR9, the receptor for the chemokine TECK. J Immunol 162(10):5671- 5.