MULTISCREEN™ STABLE CELL LINES

HUMAN RECOMBINANT CCR9 RECEPTOR

Product Information

Catalog Number:
CG1017

Lot Number:
CG1017-110609

Quantity:
1 vial (2 x 106) frozen cells

Freeze Medium:
Sigma Freezing Medium (C-6164)

Host cell:
HEK293T Gαqi5

Transfection:
Expression vector containing full-length human CCR9 cDNA (GenBank Accession Number NM_006641) with FLAG tag sequence at N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin, 250 μg/mL hygromycin

Stability:
Stable for minimum two months of continuous growth.

Data Sheet

Background: CCR9 (C-C chemokine receptor type 9) is a thymus-specific receptor for chemokine CCL25 (SCYA25 or TECK). It is an alternative co-receptor with CD4 for HIV-1 infection. Activation of the receptor may lead to potent cFLIP(L)- independent resistance to cycloheximide-induced apoptosis and modest resistance to Fas-mediated apoptosis possibly via activation of multiple signaling components involving Akt and glycogen synthase kinase 3β. Thus, dissection of signaling components involved in the CCR9-mediated antiapoptosis could be a framework for cell survival mechanisms and may provide options for therapeutic interventions.

Application: Functional assays

Figure legend: Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).

References:

Youn et al. (2002) Role of the CC chemokine receptor 9/TECK interaction in apoptosis. Apoptosis 7:271-276.

Zaballos et al. (1999) Cutting edge: identification of the orphan chemokine receptor GPR-9-6 as CCR9, the receptor for the chemokine TECK. J Immunol 162(10):5671- 5.

FOR RESEARCH USE ONLY.
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