MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT CCR5 RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Full-length human CCR5 cDNA (GenBank Accession Number NM_000579) with flag tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin, 250 μg/mL hygromycin
Background: CCR5 (chemokine (C-C motif) receptor 5) is a receptor for C-C type chemokines MIP-1-α, MIP-1-ß and CCL5. CCR5 plays a role in the control of granulocytic lineage proliferation or differentiation. It also acts as co-receptor with CD4 for primary non-syncytium-inducing strains (macrophage-tropic) of HIV-1 virus.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).
Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Deng et al. (1996) Identification of a major co-receptor for primary isolates of HIV-1. Nature 381:661-666.
Oppermann (2004) Chemokine receptor CCR5: insights into structure, function, and regulation. Cell Signal 16:1201-1210.