MULTISCREEN™ STABLE CELL LINES

HUMAN RECOMBINANT CCR5 RECEPTOR

Product Information

Catalog Number:
CG1013

Lot Number:
CG1013-060713

Quantity:
1 vial (2 x 106) frozen cells

Freeze Medium:
Sigma Freezing Medium (C-6164)

Host cell:
HEK293T

Transfection:
Full-length human CCR5 cDNA (GenBank Accession Number NM_000579) with flag tag sequence at N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin, 250 μg/mL hygromycin

Stability:
In progress

Data Sheet

Background: CCR5 (chemokine (C-C motif) receptor 5) is a receptor for C-C type chemokines MIP-1-α, MIP-1-ß and CCL5. CCR5 plays a role in the control of granulocytic lineage proliferation or differentiation. It also acts as co-receptor with CD4 for primary non-syncytium-inducing strains (macrophage-tropic) of HIV-1 virus.

Application: Functional assays

Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).

Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.

References:

Deng et al. (1996) Identification of a major co-receptor for primary isolates of HIV-1. Nature 381:661-666.

Oppermann (2004) Chemokine receptor CCR5: insights into structure, function, and regulation. Cell Signal 16:1201-1210.

FOR RESEARCH USE ONLY.
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