MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT CCR4 RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human CCR4 cDNA (GenBank accession number NM_005508.2) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin, 250 μg/mL hygromycin
Stable in culture for minimum of two months
Background: CCR4 (C-C chemokine receptor type 4) is a high affinity receptor for the C-C type chemokines CCL17/TARC and CCL22/MDC. CCR4 receptors express in the thymus, peripheral blood leukocytes and the spleen. CCR4 functions as a chemoattractant homing receptor on circulating memory lymphocytes and as a coreceptor for some primary HIV-2 isolates. In the CNS, CCR4 mediates hippocampal-neuron survival. CCR4 is one of the type 2 T helper cell (Th2) markers. Th2 cells are recruited to the asthmatic lung in response to allergen challenge and CCR4 has been therefore considered as a therapeutic target of lung inflammation, including eosinophilic inflammation of the lung and asthma. In addition, CCR4 has been reported as a potential independent and significant prognostic factor for adult T- cell leukemia/lymphoma (ATLL).
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Power and Meyer (1995) Molecular cloning and functional expression of a novel CC chemokine receptor cDNA from a human basophilic cell line. J Biol Chem 270:19495- 19500.
Imai and Baba (1997) The T cell-directed CC chemokine TARC is a highly specific biological ligand for CC chemokine receptor 4. J Biol Chem 272:15036-15042.