MULTISCREEN™ STABLE CELL LINES

HUMAN RECOMBINANT GPR120 RECEPTOR

Product Information

Catalog Number:
CA1522

Lot Number:
CA1522-061019

Quantity:
1 vial (2 x 106) frozen cells

Freeze Medium:
CellBanker 2 (Amsbio 11891)

Host cell:
HEK293T

Transfection:
Expression vector containing full-length human GPR120 cDNA (GenBank Accession Number: NM_181745.3) with FLAG tag sequence at N-terminus and ARRB2 cDNA (GenBank Accession Number NM_004313.3)

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin, 250 μg/mL hygromycin

Stability:
In progress

Data Sheet

Background: GPR120 is a G protein-coupled receptor for the long-chain free fatty acids. GPR120 mediated calcium mobilization, Erk1/Erk2 activation and GLP1 secretion. Unsaturated long-chain FFAs had a dose-dependent stimulatory effect, and α-linolenic acid was the most potent. GPR120 and GLP1 colocalized in human colonic intraepithelial neuroendocrine cells, and GPR120 may mediate dietary FFA- stimulated GLP1 secretion.

Application: Functional assays

Figure 1. Dose-dependent stimulation from arrestin recruitment upon treatment with ligand, monitored on Flexstation III. Figure 2. Receptor Expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.

References:

Fredriksson et al. (2003) Seven evolutionarily conserved human rhodopsin G protein- coupled receptors lacking close relatives. FEBS Lett 554:381-388.

Hirasawa et al. (2005) Free fatty acids regulate gut incretin glucagon-like peptide-1 secretion through GPR120. Nature Med 11:90-94.

FOR RESEARCH USE ONLY.
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